Journal: PLoS ONE

Article Title: Immunogenicity of Isogenic IgG in Aggregates and Immune Complexes

doi: 10.1371/journal.pone.0170556

Figure Lengend Snippet: (A) Adoptive transfer and immunization scheme. (B) Serum IgG anti-idiotypic antibodies directed against the Vκ of mAb 36–71 were quantified using DELFIA as described in Materials and Methods. Connected lines denote a single mouse. Data are representative of two independent experiments with n = 5 mice per group. (C) Table summarizing results from 2 experiments with n = 5 mice per group with positive titers at each time point by treatment. Both experiments had 3 mice with positive titers at d42 and 5 mice with positive titers at d63 in both the heat-aggregated and immune complex groups.

Article Snippet: For the anti-CD20 B cell depletion experiment, mice were injected i.p. with 500 μg of 5D2, an anti-mouse CD20 IgG2a/κ or control anti-ragweed monoclonal IgG2a/κ both generously donated by Genentech (San Francisco, CA) in sterile low aggregation pharmaceutical buffer 24 hours prior to i.v. adoptive transfer (day -2) of 5 x 10 4 CFSE-labeled T cells and 48 h prior to antigen injection.

Techniques: Adoptive Transfer Assay

Journal: PLoS ONE

Article Title: Immunogenicity of Isogenic IgG in Aggregates and Immune Complexes

doi: 10.1371/journal.pone.0170556

Figure Lengend Snippet: (A) Adoptive transfer and immunization scheme. Primary and secondary injections were as specified in the figure. Naïve mice were B6AF1 mice that received CA30 cells, but no primary or secondary injection. (B) Mean titration curves (above) and concentrations (below) of serum IgG anti-Id at day 21. Each point represents an experimental mouse (n = 5/group). Results representative of 2 independent experiments with n = 5 mice per group.

Article Snippet: For the anti-CD20 B cell depletion experiment, mice were injected i.p. with 500 μg of 5D2, an anti-mouse CD20 IgG2a/κ or control anti-ragweed monoclonal IgG2a/κ both generously donated by Genentech (San Francisco, CA) in sterile low aggregation pharmaceutical buffer 24 hours prior to i.v. adoptive transfer (day -2) of 5 x 10 4 CFSE-labeled T cells and 48 h prior to antigen injection.

Techniques: Adoptive Transfer Assay, Injection, Titration